Optimization of reducing sugar production from tea stalks with mild dilute alkali pretreatment by response surface methodology
23 thg 9, 2025 | 10:57

Optimization of reducing sugar production from tea stalks with mild dilute alkali pretreatment by response surface methodology



Tối ưu hóa quá trình giảm sản xuất đường từ thân cây chè bằng phương pháp xử lý kiềm loãng nhẹ bằng phương pháp bề mặt phản ứng


Current biofuel production urgently requires effective conversion of lignocellulosic biomass into fermentable sugars. This conversion process entails pretreating the biomass, which stands as one of the most costly steps, thereby emphasizing the crucial need to identify the most cost-efficient and highly effective conversion methodology. In this study, to comprehensively utilize tea stalks and enhance the enzymolysis efficiency of its biomass, a mild dilute alkali pretreatment method was employed. The production of reducing sugar was optimized by response surface methodology. 

The effects of alkaline solution concentration, solid-to-liquid ratio, and pretreatment time on the enzymatic saccharification of tea stalks were investigated. The results indicated that under the conditions of 1.5% NaOH, a solid-to-liquid ratio of 1:18 (w/v), and a reaction temperature of 80 °C for 2 h, the yield of reducing sugar from tea stalks reached 320.1 ± 1.1 mg/g, which was 3.1 times higher than that of untreated tea stalks. Moreover, structural characterization of the changes after pretreatment was performed. Scanning electron microscopy (SEM) reflected noticeable changes in surface morphology and increased fragmentation, indicating enhanced enzymatic efficiency. Fourier transform infrared spectroscopy (FT-IR) and X-ray diffraction (XRD) spectrum revealed compositional and microstructural changes caused by the mild alkali pretreatment. The mild process used in this study is a simple and effective method to produce fermentable sugars from tea stalks for value-added products.








LINK DOWNLOAD



Tối ưu hóa quá trình giảm sản xuất đường từ thân cây chè bằng phương pháp xử lý kiềm loãng nhẹ bằng phương pháp bề mặt phản ứng


Current biofuel production urgently requires effective conversion of lignocellulosic biomass into fermentable sugars. This conversion process entails pretreating the biomass, which stands as one of the most costly steps, thereby emphasizing the crucial need to identify the most cost-efficient and highly effective conversion methodology. In this study, to comprehensively utilize tea stalks and enhance the enzymolysis efficiency of its biomass, a mild dilute alkali pretreatment method was employed. The production of reducing sugar was optimized by response surface methodology. 

The effects of alkaline solution concentration, solid-to-liquid ratio, and pretreatment time on the enzymatic saccharification of tea stalks were investigated. The results indicated that under the conditions of 1.5% NaOH, a solid-to-liquid ratio of 1:18 (w/v), and a reaction temperature of 80 °C for 2 h, the yield of reducing sugar from tea stalks reached 320.1 ± 1.1 mg/g, which was 3.1 times higher than that of untreated tea stalks. Moreover, structural characterization of the changes after pretreatment was performed. Scanning electron microscopy (SEM) reflected noticeable changes in surface morphology and increased fragmentation, indicating enhanced enzymatic efficiency. Fourier transform infrared spectroscopy (FT-IR) and X-ray diffraction (XRD) spectrum revealed compositional and microstructural changes caused by the mild alkali pretreatment. The mild process used in this study is a simple and effective method to produce fermentable sugars from tea stalks for value-added products.








LINK DOWNLOAD

M_tả

M_tả

Chuyên mục:

Không có nhận xét nào: