Isolation, Screening, Identifcation of Cellulolytic Bacteria and Optimization of Factors Afecting FPUase Synthesis for Bagasse Hydrolysis (Full)
22 thg 8, 2025 | 15:42

Isolation, Screening, Identifcation of Cellulolytic Bacteria and Optimization of Factors Afecting FPUase Synthesis for Bagasse Hydrolysis (Full)



Phân lập, sàng lọc, định danh vi khuẩn phân giải cellulose và tối ưu hóa các yếu tố ảnh hưởng đến quá trình tổng hợp FPUase cho quá trình thủy phân bã mía


The screening of FPUase-synthesizing bacteria and optimization of hydrolysis conditions for alkaline pre-treated bagasse are key focuses of current research. This study systematically screened cellulose‑degrading bacteria from soil, mud, and cow liver samples, identifying HL04 as a robust FPUase‑producing strain. Based on 16S rRNA ribosome sequence, HL04 was identified as Bacillus safensis, a newly discovered microorganism capable of synthesizing FPUase. 

FPUase production conditions were optimized through single‑factor experiments analyzing fermentation time, substrate concentration, pH, and temperature. Using the Box–Behnken Design (BBD) under the response surface methodology (RSM), the study refined these factors to enhance FPUase production. The resulting second-degree polynomial model demonstrated high validity (R2 = 0.9913), and statistically significant interactions (P < 0.05). Optimal conditions—12 g/L of substrate concentration, pH 7, and a fermentation temperature of 40 °C—yielded the highest FPUase activity of 61.5 U/L.










LINK DOWNLOAD (TÀI LIỆU VIP MEMBER)



Phân lập, sàng lọc, định danh vi khuẩn phân giải cellulose và tối ưu hóa các yếu tố ảnh hưởng đến quá trình tổng hợp FPUase cho quá trình thủy phân bã mía


The screening of FPUase-synthesizing bacteria and optimization of hydrolysis conditions for alkaline pre-treated bagasse are key focuses of current research. This study systematically screened cellulose‑degrading bacteria from soil, mud, and cow liver samples, identifying HL04 as a robust FPUase‑producing strain. Based on 16S rRNA ribosome sequence, HL04 was identified as Bacillus safensis, a newly discovered microorganism capable of synthesizing FPUase. 

FPUase production conditions were optimized through single‑factor experiments analyzing fermentation time, substrate concentration, pH, and temperature. Using the Box–Behnken Design (BBD) under the response surface methodology (RSM), the study refined these factors to enhance FPUase production. The resulting second-degree polynomial model demonstrated high validity (R2 = 0.9913), and statistically significant interactions (P < 0.05). Optimal conditions—12 g/L of substrate concentration, pH 7, and a fermentation temperature of 40 °C—yielded the highest FPUase activity of 61.5 U/L.










LINK DOWNLOAD (TÀI LIỆU VIP MEMBER)

M_tả
M_tả

Chuyên mục:

Không có nhận xét nào: